期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 9, 期 7, 页码 1018-1026出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2013.03.005
关键词
AIDS; HIV-1 virus; Localized surface plasmon resonance; Biosensor; Immunosensor
资金
- National Research Foundation of Korea (NRF)
- Korea government (MEST) [2009-0080860]
- Nano/Bio Science & Technology Program of the Ministry of Education, Science, and Technology (MEST) [M10536090001-05N3609-00110]
- Ministry of Education, Science and Technology [2012-0006592]
- Original Technology Research Program for Brain Science through the National Research Foundation of Korea (NRF)
- National Research Foundation of Korea [2009-0080860] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A highly sensitive label-free immunosensor for the detection of HIV-1 is newly developed based on localized surface plasmon resonance (LSPR) method. Uniform nanopattern of circular Au-dots (10-20 nm) was fabricated on indium tin oxide (ITO) coated glass substrate by simple electrochemical deposition method. The surface of Au nanopattern was modified with HIV-1 neutralizing gp120 monoclonal antibody fragments. The modified substrate was employed to measure various concentrations of HIV-1 particles quantitatively based on the shift of longitudinal wavelength in the UV-Vis spectrum which results from the changes of local refractive index induced by specific antigen-antibody recognition events. The detection limit of the HIV-1 particles was estimated to be 200 fg/mL, which is 10 fold higher than that of previously reported virus detection method based on LSPR. Since fabricated LSPR immunosensor has high sensitivity and selectivity, it is a promising approach for biological/medical sample analysis and various kinds of virus detection. (C) 2013 Elsevier Inc. All rights reserved.
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