期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 8, 期 8, 页码 1309-1318出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2012.03.007
关键词
Microbubbles; Magnetic nanoparticles; Gene delivery; Endothelial cells; Ultrasound
资金
- German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) within DFG Research Unit FOR917
- DFG [WO1716/1-1]
- Else Kroner-Fresenius-Stiftung
Site specific vascular gene delivery for therapeutic implications is favorable because of reduction of possible side effects. Yet this technology faces numerous hurdles that result in low transfection rates because of suboptimal delivery. Combining ultrasonic microbubble technology with magnetic nanoparticle enhanced gene transfer could make it possible to use the systemic vasculature as the route of application and to magnetically trap these compounds at the target of interest. In this study we show that magnetic nanoparticle-coated microbubbles bind plasmid DNA and successfully deliver it to endothelial cells in vitro and more importantly transport their cargo through the vascular system and specifically deliver it to the vascular wall in vivo at sites where microbubbles are retained by magnetic force and burst by local ultrasound application. This resulted in a significant enhancement in site specific gene delivery compared with the conventional microbubble technique. Thus, this technology may have promising therapeutic potential.
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