期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 8, 期 4, 页码 432-439出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2011.08.001
关键词
RGD gradient; Cell adhesion; Poly(methyl methacrylate); Hydrolysis; Biotin-streptavidin
资金
- Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina (CIBER BBN), Spain
- Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya [2009 SGR 505]
- Spanish Ministry of Science and Education
- FundacionMarcelino Botin, Santander, Spain
- CICYT [CTQ2009-07758]
Cell adhesion onto bioengineered surfaces is affected by a number of variables, including the former substrate derivatization process. In this investigation, we studied the correlation between cell adhesion and cell-adhesive ligand surface concentration and organization due to substrate modification. For this purpose, Arg-Gly-Asp (RGD) gradient surfaces were created on poly(methyl methacrylate) substrates by continuous hydrolysis and were then grafted with biotin-PEG-RGD molecules. Cell culture showed that adhesion behavior changes in a nonlinear way in the narrow range of RGD surface densities assayed (2.8 to 4.4 pmol/cm(2)), with a threshold value of 4.0 pmol/cm(2) for successful cell attachment and spreading. This nonlinear dependence may be explained by nonhomogeneous RGD surface distribution at the nanometre scale, conditioned by the stochastic nature of the hydrolysis process. Atomic force microscopy analysis of the gradient surface showed an evolution of surface morphology compatible with this hypothesis. From the Clinical Editor: The authors observed by AFM nonlinear dependence of cell adhesion on RGD gradient surfaces with different surface densities. The nonlinear characteristics may be explained by non-homogeneous RGD surface distribution at the nanometer scale, conditioned by the stochastic nature of the hydrolysis process. (C) 2012 Elsevier Inc. All rights reserved.
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