期刊
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
卷 6, 期 6, 页码 738-744出版社
ELSEVIER
DOI: 10.1016/j.nano.2010.05.004
关键词
Cerium oxide nanoparticle; Phosphate ester hydrolysis; Nanoceria; Phosphatase mimetic; Dephosphorylation
资金
- M.D. Anderson Cancer Center Orlando
- University of Central Florida
- National Science Foundation [CBET: NIRT 0708172]
- National Science Foundation (NIRT) [CBET-0708172]
In an effort to characterize the interaction of cerium oxide nanoparticles (CNPs) in biological systems, we explored the reactivity of CNPs with the phosphate ester bonds of p-nitrophenylphosphate (pNPP), ATP, o-phospho-L-tyrosine, and DNA. The activity of the bond cleavage for pNPP at pH 7 is calculated to be 0.860 +/- 0.010 nmol p-nitrophenol/min/mu g CNPs. Interestingly, when CNPs bind to plasmid DNA, no cleavage products are detected. While cerium(IV) complexes generally exhibit the ability to break phosphorus-oxygen bonds, the reactions we report appear to be dependent on the availability of cerium(III) sites, not cerium(IV) sites. We investigated the dephosphorylation mechanism from the first principles and find the reaction proceeds through inversion of the phosphate group similar to an S(N)2 mechanism. The ability of CNPs to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. From the Clinical Editor: The ability of cerium oxide nanoparticles to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. This team of investigators explored the reactivity of these nanoparticles with the phosphate ester bonds of p-nitrophenylphosphate, ATP, o-phospho-L-tyrosine, and DNA. (C) 2010 Elsevier Inc. All rights reserved.
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