期刊
NANOMEDICINE
卷 8, 期 7, 页码 1101-1126出版社
FUTURE MEDICINE LTD
DOI: 10.2217/NNM.12.136
关键词
amorphous silica; cell death; fetal calf serum; inflammatory cytokines; LUDOX (R) SiO2-NPs; macrophages; monocytes; mononuclear phagocyte system; nanoparticles; reticular endothelial system; Stober SiO2-NPs
资金
- European Community's Seventh Framework Programme [201031]
- University of Padova
- NANOPHOTO
We tested whether phagocytic monocytes/macrophages are more susceptible than nonphagocytes to nanoparticle (NP) toxicity. Materials & methods: We compared in vitro cell death and proinflammatory cytokine production in human monocytes, macrophages, lymphocytes and HeLa cells due to synthetic amorphous silica (SiO2)-NPs in different serum concentrations and correlated them with cellular uptake and distribution. Results: Phagocytes were approximately ten-times more sensitive than nonphagocytes to SiO2-NPs and more effectively endocytosed SiO2-NP-serum protein nanoagglomerates, so determining their accumulation in acidic endocytic compartments well beyond a critical/cytotoxic threshold. Monocyte/macrophage death was paralleled by cytokine secretion. Conclusion: The physiological specialization of monocytes/macrophages to effectively capture NPs may expose them to the risk of catastrophic inflammatory death upon saturation of their maximal storage capacity.
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