期刊
NANOMEDICINE
卷 8, 期 11, 页码 1797-1806出版社
FUTURE MEDICINE LTD
DOI: 10.2217/nnm.12.187
关键词
cell type; cytotoxicity assay; nanoparticle; reactive oxygen species; toxicity; transmission electron microscopy
资金
- Consorzio Interuniversitario Biotecnologie
- Associazione Amici dell'Universita
- Telecom Working Capital (Bio and Nanotech) grants
Aim: The authors propose a new magnetic nanoparticle-enzyme system for cancer therapy capable of targeting the enzyme and consequently decreasing the adverse effects, meanwhile improving the patient's life quality. Materials & methods: The authors have functionalized Fe3O4 nanoparticles with 3-amino-propyltriethoxysilane (APTES) and conjugated it to yeast D-amino acid oxidase (DAAO) by coupling this with glutaraldehyde. Results & conclusion: The authors have tested the Fe3O4-APTES-DAAO system on three tumor cell lines. Exposed cells show, at the electron microscope level, nanoparticles on the surface of the plasma membrane and inside endocytic vesicles. Fe3O4-APTES-DAAO caused a substantial decrease of cell viability greatly augmented when D-alanine, a DAAO substrate, was added. Fe3O4-APTES-DAAO was demonstrated to be more effective than free DAAO, confirming the validity of the system in cancer therapy. Original submitted 27 March 2012; Revised submitted 20 September 2012; Published online 5 February 2013
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