期刊
NANOMEDICINE
卷 8, 期 6, 页码 891-902出版社
FUTURE MEDICINE LTD
DOI: 10.2217/NNM.12.142
关键词
hyperthermia; magnetite nanoparticle; melanoma; T-cell receptor; tumor-infiltrating lymphocyte
资金
- Ministry of Health, Labour and Welfare of Japan [H21-nano-006]
- Grants-in-Aid for Scientific Research [22591429, 22591228] Funding Source: KAKEN
Aim: Accumulating evidence has indicated that hyperthermia using magnetite nanoparticles induces anti-tumor immunity. This study investigated the diversity of T-cell receptors (TCRs) in tumor-infiltrating lymphocytes after hyperthermia using magnetite nanoparticles. Materials & methods: Functionalized magnetite nanoparticles, N-propionyl-4-S-cysteaminylphenol (NPrCAP)/magnetite, were synthesized by conjugating the melanogenesis substrate NPrCAP with magnetite nanoparticles. NPrCAP/magnetite nanoparticles were injected into B16 melanomas in C57BL/6 mice, which were subjected to an alternating magnetic field for hyperthermia treatment. Results: Enlargement of the tumor-draining lymph nodes was observed after hyperthermia. The TCR repertoire was restricted in tumor-infiltrating lymphocytes, and expansion of V beta 11(+) T cells was preferentially found. DNA sequences of the third complementarity-determining regions revealed the presence of clonally expanded T cells. Conclusion: These results indicate that the T-cell response in B16 melanomas after hyperthermia is dominated by T cells directed toward a limited number of epitopes and that epitope-specific T cells frequently use a restricted TCR repertoire.
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