A label-free colorimetric sensor for sulfate based on the inhibition of peroxidase-like activity of cysteamine-modified gold nanoparticles

A simple label-free colorimetric sensor for sulfate has been devised based on the analyte-induced shielding of peroxidase-like activity of cysteamine-capped gold nanoparticles (cyst-AuNPs). Aggregation triggered by sulfate through hydrogen bond and electrostatic interaction gave rise to a remarkable decrease of the catalytic activity of AuNPs. By coupling with 3,3',5,5'-tetramethylbenzidine (TMB)-H2O2 chromogenic reaction, a novel colorimetric sensor for detection of sulfate was established. Based on the signal amplification effect of the catalytic reaction, a detection limit as low as 0.16 mu M was obtained by virtue of UV-vis spectroscopy or 4 mu M with the naked-eye observation. The as-proposed sensor exhibited an excellent selectivity over other common anions and metallic ions. In view of these characteristics, such sensing probe features simplicity, cost-effectiveness, and naked-eye screening. These advantages make this sensor a powerful protocol for the quantitative detection of sulfate in water samples with satisfactory results. (C) 2015 Elsevier B.V. All rights reserved.