期刊
NANO RESEARCH
卷 2, 期 6, 页码 448-461出版社
TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-009-9041-8
关键词
Silica nanoparticle; flow cytometry; aptamer; cell detection; polyethylene glycol; fluorophore
类别
资金
- NIH National Cancer Institute [R21CA122648]
- ONR [N00014-07-1-0982]
- State of Florida Center of Excellence
- Department d'Universitats, Recerca i Societat de la Informacio de la Generalitat de Catalunya, Spain
Early and accurate diagnosis and treatment of cancer depend on rapid, sensitive, and selective detection of tumor cells. Current diagnosis of cancers, especially leukemia, relies on histology and flow cytometry using single dye-labeled antibodies. However, this combination may not lead to high signal output, which can hinder detection, especially when the probes have relatively weak affinities or when the receptor is expressed in a low concentration on the target cell surface. To solve these problems, we have developed a novel method for sensitive and rapid detection of cancer cells using dye-doped silica nanoparticles (NPs) which increases detection sensitivity in flow cytometry analyses between 10- and 100-fold compared to standard methods. Our NPs are similar to 60 nm in size and can encapsulate thousands of individual dye molecules within their matrix. We have extensively investigated surface modification strategies in order to make the NPs suitable for selective detection of cancer cells using flow cytometry. The NPs are functionalized with polyethylene glycol (PEG) to prevent nonspecific interactions and with neutravidin to allow universal binding with biotinylated molecules. By virtue of their reliable and selective detection of target cancer cells, these NPs have demonstrated their promising usefulness in conventional flow cytometry. Moreover, they have shown low background signal, high signal enhancement, and efficient functionalization, either with antibody- or aptamer-targeting moieties.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据