期刊
NANO LETTERS
卷 18, 期 10, 页码 6633-6637出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.8b03342
关键词
Plasmon ruler; single molecule; Hsp90; protein dynamics; nonergodicity
类别
资金
- ERC [259640, 681891]
- DFG fellowships through the Excellence Initiative by the Graduate School Materials Science in Mainz [GSC 266]
We use plasmon rulers to follow the conformational dynamics of a single protein for up to 24 h at a video rate. The plasmon ruler consists of two gold nanospheres connected by a single protein linker. In our experiment, we follow the dynamics of the molecular chaperone heat shock protein 90 (Hsp90), which is known to show open and closed conformations. Our measurements confirm the previously known conformational dynamics with transition times in the second to minute time scale and reveals new dynamics on the time scale of minutes to hours. Plasmon rulers thus extend the observation bandwidth 3-4 orders of magnitude with respect to single-molecule fluorescence resonance energy transfer and enable the study of molecular dynamics with unprecedented precision.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据