期刊
NANO LETTERS
卷 14, 期 12, 页码 6850-6856出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl504358j
关键词
Stem cell niche; bionanofiber; phage display; peptides; viruses; topography
类别
资金
- Oklahoma Center for Adult Stem Cell Research [434003]
- National Science Foundation [CMMI-1234957, CBET-0854414, CBET-0854465, DMR-0847758]
- National Institutes of Health [1R01DE015633, 1R21EB015190]
- Department of Defense Peer Reviewed Medical Research Program [W81XWH-12-1-0384]
- Oklahoma Center for the Advancement of Science and Technology [HR14-160]
- National High Technology Research and Development Program [2013AA102507]
- Zhejiang Provincial Natural Science Foundation of China [LZ12C17001]
- National Natural Science Foundation of China [20804037, 21172194]
- Silkworm Industry Science and Technology Innovation Team [2011R50028]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [847758] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1234957] Funding Source: National Science Foundation
Here we report the design of a unique matrix, assembled from engineered M13 phage bionanofibers with specific cues of nanotopographies and versatile signal peptides to simulate native niche for directing the fate of induced pluripotent stem cells (iPSCs). By independently varying the peptide sequences and nanotopographies, we find that the resident iPSCs on the phage matrix are first differentiated into mesenchymal progenitor cells (MPCs), which are further differentiated into osteoblasts in the absence of osteogenic supplements due to the elongation induced by phage nanofibers. The phage-based matrix represents not only a biomimetic stem cell niche enabling independently varying biochemical and biophysical cues in one system but also a substrate for generating a safe and efficient cell source for tissue engineering.
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