期刊
NANO LETTERS
卷 14, 期 10, 页码 5539-5546出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl501912g
关键词
Nanopatterning; receptor clustering; molecular tension fluorescence microscopy; focal adhesion; mechanotransduction; integrin
类别
资金
- NIH [R01-GM097399]
- Alfred P. Sloan Research Fellowship
- Camille-Dreyfus Teacher-Scholar Award
- NSF [1353939, 1350829]
- Max Planck Society
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1350829] Funding Source: National Science Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1353939] Funding Source: National Science Foundation
Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.
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