期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 209, 期 -, 页码 770-774出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.12.015
关键词
Biosensor; Nanostructuration; Polyoxometalate; Benzo[a]pyrene; QCM-D
资金
- ANR, Agence Nationale de la Recherche (France)
- DFG, Deutsche Forschungsgemeinschaft (Germany), ANR-DFG program [ANR-11-INTB-1013]
- Agence Nationale de la Recherche (ANR) [ANR-11-INTB-1013] Funding Source: Agence Nationale de la Recherche (ANR)
We report the design of a polyoxometalate-nanostructured immunosensor for benzo[a]pyrene (B[a]P) detection. The organic-inorganic hybrid polyoxometalate (POM) (NBu4)(3)[PW11 O-39 {(SiC6H4NH2)(2)O}] carrying two amine functions was covalently attached to a functionalized gold substrate to achieve a nanometric organization of amine groups at its surface. Pyrenebutyric acid (PBA) was subsequently grafted to amine groups to create the sensing layer. The detection of B[a]P in the indirect competitive format was carried out using a monoclonal anti-B[a]P antibody whose binding to the immunoprobe was monitored with a quartz crystal microbalance with dissipation measurement (QCM-D). The performances of the POM-nanostructured biosensor were compared to a reference sensor constructed from a cysteamine self-assembled monolayer. QCM-D measurements displayed significant input from POM-nanostructuration. Both the accessibility of the analogue on the surface and the analytical performances were enhanced showing a promising effect of this strategy of nanostructuration for the biosensing of small molecules. (C) 2014 Elsevier B.V. All rights reserved.
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