期刊
NANO LETTERS
卷 14, 期 8, 页码 4197-4204出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl4042565
关键词
Nanomembraties; rolled-up nanotechnology; mitosis; 3D cell culture scaffold; spatial confinement; chromosome segregation errors
类别
资金
- European Research Council under the European Union/ERC [311529]
- Volkswagen Foundation [86 362]
- Deutsche Forschungsgemeinschaft through the Research Unit FG 1713
- FEBS Return-to-Europe fellowship
- European Research Council (ERC)
- Alexander von Humboldt Foundation
- U.S. National Science Foundation [CMMI 1200241]
- Cancer Research U.K.
- ERC
- European Community Seventh Framework Programme
- Cancer Research UK [11224] Funding Source: researchfish
We use micropatterning and strain engineering to encapsulate single living mammalian cells into transparent tubular architectures consisting of three-dimensional (3D) rolled-up nanomembranes. By using optical microscopy, we demonstrate that these structures are suitable for the scrutiny of cellular dynamics within confined 3D-microenvironments. We show that spatial confinement of mitotic mammalian cells inside tubular architectures can perturb metaphase plate formation, delay mitotic progression, and cause chromosomal instability in both a transformed and nontransformed human cell line. These findings could provide important clues into how spatial constraints dictate cellular behavior and function.
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