期刊
NANO LETTERS
卷 14, 期 5, 页码 2709-2714出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl5006349
关键词
Graphene; biosensor; field effect transistor; mu-opioid receptor; computational protein design
类别
资金
- National Science Foundation Accelerating Innovation in Research program [AIR ENG-1312202]
- Nano/Bio Interface Center NSF NSEC [DMR08-32802]
- FAER (Foundation for Anesthesia Education and Research)
- NIH [K08-GM-093115]
- GROFF
- Department of Anesthesiology
- Critical Care at the University of Pennsylvania
- Penn LRSM MRSEC [NSF DMR-1120901]
- Leading Foreign Research Institute Recruitment Program of NRF [0409-20100156]
- FPRD of BK21 through the MEST, Korea
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1312202] Funding Source: National Science Foundation
We have developed a novel, all-electronic biosensor for opioids that consists of an engineered mu-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of similar to 1500 cm(2) V-1 s(-1) and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.
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