期刊
NANO LETTERS
卷 14, 期 5, 页码 2831-2836出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl500841n
关键词
DNA self-assembly; single-molecule; photobleaching; plasmonics; gold nanoparticle
类别
资金
- European Research Council (ERC) [EU 261162]
- DAAD
- ANCyPT [PICT2010-2511]
The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules non-fluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching.
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