期刊
NANO LETTERS
卷 13, 期 7, 页码 3347-3351出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl401633y
关键词
Whispering gallery mode; hybrid microcavity; surface plasmons; single protein detection; BSA protein; label-free detection; nanoshell; thyroid cancer maker
类别
资金
- National Science Foundation (NSF) [CBET 0933531]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0933531] Funding Source: National Science Foundation
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [1303499] Funding Source: National Science Foundation
Recently we reported the detection and sizing of the smallest RNA virus MS2 with a mass of 6 ag from the resonance frequency shift of a whispering gallery mode-nanoshell hybrid resonator (WGM-h) upon adsorption on the nanoshell and anticipated that single protein above 0.4 ag should be detectable but with considerably smaller signals. Here, we report the detection of single thyroid cancer marker (Thyroglobulin, Tg) and bovine serum albumin (BSA) proteins with masses of only 1 ag and 0.11 ag (66 kDa), respectively. However, the wavelength shifts are enhanced beyond those anticipated in our earlier work by 240% for Tg and 1500% for BSA. This surprising sensitivity is traced to a short-range reactive field near the surface of our Au nanoshell receptor due to intrinsic random bumps of protein size, leading to an unanticipated increase in sensitivity to single protein, which grows larger as the protein diminishes in size. As a consequence of the largest signal-to-noise ratio in our BSA experiments (S/N approximate to 13), we conservatively estimated a new protein limit of detection for our WGM-h of 5 kDa.
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