期刊
NANO LETTERS
卷 13, 期 6, 页码 2463-2470出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl400448n
关键词
Gold nanoparticles; surface-enhanced Raman scattering; cellular imaging; nuclear localization signal; cellular distinction
类别
资金
- EPSRC (Engineering and Physical Sciences Research Council), United Kingdom [EP/H028757/1, EP/G060649/1]
- EPSRC [EP/K028510/1, EP/H028757/2, EP/H028757/1, EP/G060649/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H028757/2, EP/H028757/1, EP/G060649/1, EP/K028510/1] Funding Source: researchfish
Distinction between closely related and morphologically similar cells is difficult by conventional methods especially without labeling. Using nuclear-targeted gold nanoparticles (AuNPs) as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analyzed using principal component analysis that allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear-targeted AuNPs and SERS imaging provides label-free and noninvasive characterization that can play a vital role in identifying cell types in biomedical stem cell research.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据