期刊
NANO LETTERS
卷 13, 期 12, 页码 6002-6008出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl403201a
关键词
Nanowire; cell membrane; penetration mechanism; mechanical model
类别
资金
- National Institutes of Health [R21 MH091471]
- United States Israel Binational Science Foundation [2007425]
- Coulter Foundation
- Stanford BioX-Neuroventures Award
- China Scholarship Council [2009638027]
- NSF
Direct access into cells' interiors is essential for biomolecular delivery, gene transfection, and electrical recordings yet is challenging due to the cell membrane barrier. Recently, molecular delivery using vertical nanowires (NWs) has been demonstrated for introducing biomolecules into a large number of cells in parallel. However, the microscopic understanding of how and when the nanowires penetrate cell membranes is still lacking, and the degree to which actual membrane penetration occurs is controversial. Here we present results from a mechanical continuum model of elastic cell membrane penetration through two mechanisms, namely through impaling as cells land onto a bed of nanowires, and through adhesion-mediated penetration, which occurs as cells spread on the substrate and generate adhesion force. Our results reveal that penetration is much more effective through the adhesion mechanism, with NW geometry and cell stiffness being critically important. Stiffer cells have higher penetration efficiency, but are more sensitive to NW geometry. These results provide a guide to designing nanowires for applications in cell membrane penetration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据