期刊
NANO LETTERS
卷 13, 期 2, 页码 781-785出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl304492y
关键词
DNA origami; superresolution; single molecules; nanotechnology; fluorescence; biophysics
类别
资金
- (SiMBA) of the European Research Council
- Biophotonics IV program of the Federal Ministry of Education and Research (BMBF) [13N11461]
- Biophotonics IV program of the Federal Ministry of Education and Research (VDI) [13N11461]
- German Research Foundation [DFG Ti329/6-1, LI1743/12-1]
- Center for NanoScience, Munchen
Nanopillars are promising nanostructures composed of various materials that bring new functionalities for applications ranging from photovoltaics to analytics. We developed DNA nanopillars with a height of 220 nm and a diameter of similar to 14 nm using the DNA origami technique. Modifying the base of the nanopillars with biotins allowed selective, upright, and rigid immobilization on solid substrates. With the help of site-selective dye labels, we visualized the structure and determined the orientation of the nanopillars by three-dimensional fluorescence superresolution microscopy. Because of their rigidity and nanometer-precise addressability, DNA origami nanopillars qualify as scaffold for the assembly of plasmonic devices as well as for three-dimensional superresolution standards.
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