期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 215, 期 -, 页码 577-583出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.04.011
关键词
Colorimetry; Flatbed scanner; Organophosphorus (OP) pesticides; Polycarbonate (PC); Color space; Color intensity
资金
- Natural Science and Engineering Research Council (NSERC) of Canada
- Natural Science Foundation of China [61174010]
- Shanxi Provincial Government (100-talents program)
- Shanxi Province International Cooperation Project [2012081043]
- China Scholarship Council [2013-038]
Herein we report a scanner-readable plastic microchip for the colorimetric quantitation of organophosphorus (OP) pesticides in food products. The detection principle relies on the irreversible activity inhibition of acetylcholinesterase (AChE) by OP pesticides; AChE catalytically hydrolyzes acetylthiocholine (ATCh) to thiocholine, which in-turn reacts with dithiobis-nitrobenzoic acid (DTNB) to produce a yellow colored product (deprotonated 5-thio-2-nitrobenzoic acid, TNB). The yellow color diminishes upon increasing the concentration of the OP pesticides. In particular, we have fabricated a simple plastic microchip consisting of a transparent polydimethylsiloxane (PDMS) plate with embedded micro-channels (for the colorimetric reaction) attached to a polycarbonate (PC) base plate. A standard flatbed office scanner was used to image the colorimetric reaction at real time, and a commercial photo editing software to read the yellow intensity of the captured images. The detection limit for malathion (one of the most popularly used OP pesticides nowadays) was determined to be 100 ppb, which is below the food safety limits and comparable with conventional spectroscopic detection. The simplicity of the device fabrication and operation of this colorimetric protocol augments its potential for on-site food safety analysis in place of rather expensive and complex spectrometer-based methods. (C) 2015 Elsevier B.V. All rights reserved.
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