期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 221, 期 -, 页码 341-349出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.06.091
关键词
Odorant binding protein (OBP); Nanocup arrays; Localized surface plasmon resonance (LSPR); Small odorant molecules; Nitro-explosives
资金
- National Natural Science Foundation of China [81371643, 31372254]
- Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars [LR13H180002]
Odorant binding proteins (OBPs) are key soluble sensitive proteins for odor detections in animal olfactory systems. Here, we present a nanosensor based localized surface plasmon resonance (LSPR) to monitor binding of small odorant molecules to OBPs from honeybees. The binding of odorants molecules to OBPs, can be quantified by nanocup arrays from 10 nM to I mM, with a high refractive index sensing of LSPR wavelength shift in transmission spectra. Linear dose-dependent behavior can be observed in relationship of LSPR wavelength versus analyte concentrations. More than floral odorants, this type of bio-functionalized nanocups also showed potential applications in explosive detections for nitro-compounds such as 2,4,6-trinitrotoluene, 2,4-dinitrotoluene and 3-mononitrotoluene. This study demonstrated a LSPR device to monitor interactions between small molecules and proteins, which provided a great biosensor platform for healthcare diagnosis, environment monitoring and food analysis. (C) 2015 Elsevier B.V. All rights reserved.
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