期刊
NANO LETTERS
卷 12, 期 8, 页码 4164-4171出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl301726g
关键词
Nanobiosensor; cancer detection; immunosensing; gold nanoparticles; hydrogen evolution reaction; electrocatalysis
类别
资金
- MICINN (Madrid) [PIB2010JP-00278, IT2009-0092]
- E.U. [246513]
- NATO Science for Peace and Security Programme [SfP 983807]
- ICREA Funding Source: Custom
Here we present a new strategy for a simple and fast detection of cancer circulating cells (CTCs) using nanoparticles. The human colon adenocarcinoma cell line (Caco2) was chosen as a model CTC. Similarly to other adenocarcinomas, colon adenocarcinoma cells have a strong expression of EpCAM, and for this reason this glycoprotein was used as the capture target. We combine the capturing capability of anti-EpCAM functionalized magnetic beads (MBs) and the specific labeling through antibody-modified gold nanoparticles (AuNPs), with the sensitivity of the AuNPs-electrocatalyzed hydrogen evolution reaction (HER) detection technique. The fully optimized process was used for the electrochemical detection of Caco2 cells in the presence of monocytes (THP-1), other circulating cells that could interfere in real blood samples. Therefore we obtained a novel and simple in situ-like sensing format that we applied for the rapid quantification of AuNPs-labeled CTCs in the presence of other human cells.
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