期刊
NANO LETTERS
卷 13, 期 1, 页码 153-158出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl3037068
关键词
Silicon nanowire; sandwich assembly; live cell assay; enzyme activity
类别
资金
- National Research Foundation of Korea
- Korean Ministry of Education, Science and Technology
- KIST
- NIH [5DP1OD003893-03]
- NIH CEGS Award [1P50HG006193-01]
Developing a detailed understanding of enzyme function in the context of an intracellular signal transduction pathway requires minimally invasive methods for probing enzyme activity in situ. Here, we describe a new method for monitoring enzyme activity in living cells by sandwiching live cells between two vertical silicon nanowire (NW) arrays. Specifically, we use the first NW array to immobilize the cells and then present enzymatic substrates intracellularly via the second NW array by utilizing the NWs' ability to penetrate cellular membranes without affecting cells' viability or function. This strategy, when coupled with fluorescence microscopy and mass spectrometry, enables intracellular examination of protease, phosphatase, and protein kinase activities, demonstrating the assay's potential in uncovering the physiological roles of various enzymes.
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