期刊
NANO LETTERS
卷 12, 期 11, 页码 5815-5820出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl303163y
关键词
Nanopillar; cell membrane; cell-nanostructure interface; transmission electron microscopy
类别
资金
- NSF [1055112]
- NIH [NS057906]
- Searle Scholar award
- Packard Science and Engineering Fellowship
- National Defense Science and Engineering Graduate Fellowship
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1055112] Funding Source: National Science Foundation
Vertically aligned nanopillars can serve as excellent electrical, optical. and mechanical platforms. for biological studies. However, revealing the nature of the interface between the cell and the nanopillar is Very challenging. In particular, a matter of debate is whether the cell Membrane remains intact around the nanopillar. Here we present a detailed characterization of the cell-nanopillar interface by transmission electron microscopy.. We examined cortical neurons growing on nanopillars with diameter 50-500 nm and heights 0.5-2 mu m. We found that on nanopillars, less than 300 am in diameter, the cell Membrane wraps around the entirety of the nanopillar Without the nanopillar penetrating into the interior of the cell. On the other hand, the cell sits. on top of arrays of larger, closely Spaced nanopillars. We also observed that the membrane-surface gap Of both cell bodies and neurites is smaller for nanopillars than for a flat substrate. These results support a tight interaction between the cell membrane and the nanopillars and previous findings of excellent sealing in electrophysiology recordings using nanopillar electrodes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据