期刊
NANO LETTERS
卷 12, 期 10, 页码 5192-5195出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl302302e
关键词
RNA nanotechnology; self-assembly; siRNA; chemical modifications; transcription; T7 RNA polymerase
类别
资金
- Intramural Research Program of the NIH, National Cancer Institute, Frederick National Laboratory for Cancer Research, Center for Cancer Research
- NIH [R01GM-079604]
We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover, the unpurified transcription mixture can be used for functional ex vivo pilot experiments.
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