期刊
NANO LETTERS
卷 12, 期 12, 页码 6322-6327出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl303421h
关键词
QD; cytosolic delivery; microfluidic device; single QD tracking
类别
资金
- NIH [5-U54-CA151884-03, 5R01CA126642-02, RC1 EB011187-02]
- Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems [NSF-0070319, NIH GM68762]
- DCIF at MIT [CHE-9808061, DBI-9729592]
- NCI through the Cancer Center Support (core) at MIT [P30-CA14051]
The ability to straightforwardly deliver engineered nanoparticles into the cell cytosol with high viability will vastly expand the range of biological applications. Nanoparticles could potentially be used as delivery vehicles or as fluorescent sensors to probe the cell. In particular, quantum dots (QDs) may be used to illuminate cytosolic proteins for long-term microscopy studies. Whereas recent advances have been successful in specifically labeling proteins with QDs on the cell membrane, cytosolic delivery of QDs into live cells has remained challenging. In this report, we demonstrate high throughput delivery of QDs into live cell cytoplasm using an uncomplicated microfluidic device while maintaining cell viabilities of 80-90%. We verify that the nanoparticle surface interacts with the cytosolic environment and that the QDs remain nonaggregated so that single QDs can be observed.
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