期刊
NANO LETTERS
卷 12, 期 5, 页码 2530-2536出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl300739n
关键词
Photomelting; hybridization stringency; optical modulation; plasmonic nanoparticles; single nucleotide polymorphism; sensing
类别
资金
- Air Force Office of Scientific Research (AFOSR) [FA9550-10-1-0474]
- NSF [CMMI-0709131]
- Camille Dreyfus Teacher-Scholar Awards Program
- NSERC of Canada
We describe a new class of stimulus-responsive DNA-functionalized gold nanoparticles that incorporate azobenzene-modified oligonucleotides. Beyond the classic directed assembly and sensing behaviors associated with oligonucleotide-modified nanoparticles, these particles also exhibit reversible photoswitching of their assembly behavior. Exposure to UV light induces a trans-cis isomerization of the azobenzene which destabilizes the DNA duplex, resulting in dissociation of the nanoparticle assemblies. The isomerization is reversible upon exposure to blue light, resulting in rehybridization and reassembly of the DNA-linked nanoparticle clusters. We show that perfectly complementary and partially mismatched strands exhibit clearly distinguishable photoinduced melting properties, and we demonstrate that photon dose can thus be used in place of temperature or ionic strength to control hybridization stringency with the ability to discriminate single-base mismatches.
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