期刊
NANO LETTERS
卷 12, 期 9, 页码 4992-4996出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl302619p
关键词
T-cell activation; patterned antigen surface; immobilized antigen; cell interaction with SAM; X-ray photoelectron spectroscopy of SAMS
Nanoscale organization of surface ligands often has a critical effect on cell surface interactions. We have developed an experimental system that allows a high degree of control over the 2-D spatial distribution of ligands. As a proof of concept, we used the developed system to study how T-cell activation is independently affected by antigen density and antigen amount per cell. Arrays of submicrometer gold islands at varying surface coverage were defined on silicon by electron beam lithography (EBL). The gold islands were functionalized with alkanethiol self-assembled monolayers (SAMs) containing a small antigen, 2,4,6-trinotrophenyl (TNP), at various densities. Genetically engineered T-cell hybridomas expressing TNP-specific chimeric T-cell antigen receptor (CAR) were cultured on the SAMs, and their activation was assessed by IL-2 secretion and CD69 expression. It was found that, at constant antigen density, activation increased monotonically with the amount of antigen, while at constant antigen amount activation was maximal at an intermediate antigen density, whose value was independent of the amount of antigen.
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