期刊
NANO LETTERS
卷 11, 期 5, 页码 2157-2163出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl2008218
关键词
Viscosity; diffusion; cytoplasm; complex liquids; scaling
类别
资金
- EU [TEAM/2008-2/2]
- Ministry of Science and Higher Education
- European Union through Innovative Economy [POIG.02.02.00-00-025/09]
- Human Frontier Science Program Organization
- Polish Academy of Sciences
We present a scaling formula for size-dependent viscosity coefficients for proteins, polymers, and fluorescent dyes diffusing in complex liquids. The formula was used to analyze the mobilities of probes of different sizes in HeLa and Swiss 3T3 mammalian cells. This analysis unveils in the cytoplasm two length scales: (i) the correlation length xi (approximately 5 nm in HeLa and 7 nm in Swiss 3T3 cells) and (ii) the limiting length scale that marks the crossover between nano- and macroscale viscosity (approximately 86 nm in HeLa and 30 nm in Swiss 3T3 cells). During motion, probes smaller than xi experienced matrix viscosity: eta(matrix) approximate to 2.0 mPa.s for HeLa and 0.88 mPa.s for Swiss 3T3 cells. Probes much larger than the limiting length scale experienced macroscopic viscosity, eta(macro) approximate to 4.4 x 10(-2) and 2.4 x 10(-2) Pa.s for HeLa and Swiss 3T3 cells, respectively. Our results are persistent for the lengths scales from 0.14 nm to a few hundred nanometers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据