期刊
NANO LETTERS
卷 11, 期 2, 页码 746-750出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl1038874
关键词
Alpha-hemolysin; DNA sequencing; nanopore; protein engineering; DNA translocation
类别
资金
- National Institutes of Health
Protein nanopores may provide a cheap and fast technology to sequence individual DNA molecules. However, the electrophoretic translocation of ssDNA molecules through protein nanopores has been too rapid for base identification. Here, we show that the translocation of DNA molecules through the alpha-hemolysin protein nanopore can be slowed controllably by introducing positive charges into the lumen of the pore by site directed mutagenesis. Although the residual ionic current during DNA translocation is insufficient for direct base identification, we propose that the engineered pores might be used to slow down DNA in hybrid systems, for example, in combination with solid-state nanopores.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据