期刊
NANO LETTERS
卷 11, 期 8, 页码 3431-3434出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl2018838
关键词
Biosensing; plasmonic resonator; in situ immunoassay; cytokine; T cell; nanoimprint lithography (NIL)
类别
资金
- National Institutes of Health (NIH), Nanomedicine Development Center (Center for Cell Control) [PN2EY018228]
- NIH
- National Science Foundation (NSF) Nanoscale Science and Engineering Center (SINAM) [CMMI-0751621]
- Directorate For Engineering [0751621] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn [0751621] Funding Source: National Science Foundation
Local extracellular signaling is central for cellular interactions and organizations. We report a novel sensing technique to interrogate extracellular signaling at the subcellular level. We developed an in situ immunoassay based on giant optical enhancement of a tunable nano-plasmonic-resonator array fabricated by nanoimprint lithography. Our nanoplasmonic device significantly increases the signal-to-noise ratio to enable the first time submicrometer resolution quantitative mapping of endogenous cytokine secretion. Our study shows a markedly high local interleukin-2 (IL-2) concentration within the immediate vicinity of the cell which finally validates a decades-old hypothesis on autocrine physiological concentration and spatial range. This general sensing technique can be applied for a broad range of cellular communication studies to improve our understanding of subcellular signaling and function.
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