期刊
NANO LETTERS
卷 11, 期 8, 页码 3334-3340出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl201707d
关键词
Nanopore; bilayer; OmpF; antibiotics; hybrid nanopore; single molecule
类别
资金
- Nanoscience E+ initiative (through DFG) [WI 2279/18-1]
- EPRSC, through the Physics of Medicine Initiative, University of Cambridge
- Deutsche Telekom Foundation
- Boehringer Ingelheim Fonds
- DFG
- EPSRC [EP/H006672/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H006672/1] Funding Source: researchfish
We developed a new, simple and robust approach for rapid screening of single molecule interactions with protein channels. Our glass nanopipets can be fabricated simply by drawing glass capillaries in a standard pipet puller, in a matter of minutes, and do not require further modification before use. Giant unilamellar vesicles break when in contact with the tip of the glass pipet and form a supported bilayer with typical seal resistances of similar to 140 G Omega, which is stable for hours and at applied potentials up to 900 mV. Bilayers can be formed, broken, and reformed more than 50 times using the same pipet enabling rapid screening of bilayers for single protein channels. The stability of the lipid bilayer is significantly superior to that of traditionally built bilayers supported by Teflon membranes, particularly against perturbation by electrical and mechanical forces. We demonstrate the functional reconstitution of the E. coli porin OmpF and a-hemolysin in a glass nanopipet supported bilayer. Interactions of the antibiotic enrofloxacin with the OmpF channel have been studied at the single-molecule level, demonstrating the ability of this method to detect single molecule interactions with protein channels. High-resolution conductance measurements of protein channels can be performed with low sample and buffer consumption. Glass nanopipet supported bilayers are uniquely suited for single-molecule studies as they are more rigid and the lifetime of a stable membrane is on the scale of hours, closer to that of natural cell membranes.
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