期刊
NANO LETTERS
卷 11, 期 4, 页码 1401-1405出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl1024062
关键词
Phospholipase A2/gold nanoparticles; liposomes; polypeptides; helix-loop-helix
类别
资金
- Knut and Alice Wallenberg Foundation
- EPSRC
- ERC
- World Gold Council
- EPSRC [EP/E007627/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/E007627/1] Funding Source: researchfish
A flexible nanoparticle-based phospholipase (PL) assay is demonstrated in which the enzymatic substrate is decoupled from the nanoparticle surface. Liposomes are loaded with a polypeptide that is designed to heteroassociate with a second polypeptide immobilized on gold nanoparticies. Release of this polypeptide from the liposornes, triggered by PL, induces a folding-dependent nanoparticle bridging aggregation. The colorimetric response from this aggregation enables straightforward and continuous detection of PL in the picomolar range. The speed, specificity, and flexibility of this assay make it appropriate for a range of applications, from point of care diagnostics to high throughput pharmaceutical screening.
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