期刊
NANO LETTERS
卷 11, 期 10, 页码 4074-4078出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl201225r
关键词
Two photon; quantum dot; FIONA; holographic excitation; breast cancer; EGFR
类别
资金
- NIH [GM086214, R01GM082837]
- NSF [DBI 0649779, EAGER 0968976, 082265]
- HFSP [RGY 70/2008]
- CR-UK EPSRC
We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9 x 9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.
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