期刊
NANO LETTERS
卷 11, 期 9, 页码 3970-3973出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl202290w
关键词
Microscopy; two-color; superresolution; photoswitching; green fluorescent protein; RSFP
类别
资金
- Deutsche Forschungsgemeinschaft through the DFG-Research Center for Molecular Physiology of the Brain
- Deutsche Forschungsgemeinschaft
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
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