期刊
NANO LETTERS
卷 10, 期 9, 页码 3620-3627出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl101939e
关键词
Viral assembly; nanotechnology; nanostructure; nanobiotechnology; nanomedicine; bacteriophage phi29; liposomes; ion channel; rectification; DNA packaging; nanomotors; membrane channel
类别
资金
- NIH Nanomedicine Development Center [PN2 EY 018230]
- [GM059944]
Linear double-stranded DNA (dsDNA) viruses package their genome into a procapsid using an ATP-driven nanomotor. Here we report that bacteriophage phi29 DNA packaging motor exercises a one-way traffic property for dsDNA translocation from N-terminal entrance to C-terminal exit with a valve mechanism in DNA packaging, as demonstrated by voltage ramping, electrode polarity switching, and sedimentation force assessment. Without the use of gating control as Found in other biological channels, the observed single direction dsDNA transportation provides a novel system with a natural valve to control dsDNA loading and gene delivery in bioreactors, liposomes, or high throughput DNA sequencing apparatus.
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