期刊
NANO LETTERS
卷 10, 期 5, 页码 1677-1681出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl100004m
关键词
Single-walled carbon nanotubes (SWNTs); subcellular localization; transmembranal mechanism; mitochondria; lysosomes
类别
资金
- National Basic Research Program of China [2010CB732602]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0829]
- NSFC-Guangdong Joint Funds of China [U0931005]
- National Natural Science Foundation of China [30870676, 30800261]
- U.S. National Institutes of Health, National Center for Research Resources [P20 RR016478]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016478] Funding Source: NIH RePORTER
Translocation and localization of single-walled carbon nanotubes (SWNTs) in normal and cancerous cells have significant biomedical implications. In this study, SWNTs functionalized with different biomolecules in cells were observed with confocal laser scanning microscopy. Functionalized with PL-PEG, SWNTs were found to localize exclusively in mitochondria of both tumor and normal cells due to mitochondrial transmembrane potential, but they were found mainly in lysosomes of macrophages due to phagocytosis. However, when conjugated with different molecules, the subcellular localization of the surface-modified SWNT-PL-PEG depended on how SWNTs enter the cells: inside mitochondria if crossing cell membrane or inside lysosomes if being endocytosized. We also show that mitochondrial SWNT-PL-PEG, when irradiated with a near-infrared light, can induce cell apoptosis due to mitochondria! damages. These findings provide a better mechanistic understanding of cellular localization of SWNTs, which could lead to advanced biomedical applications such as the design of molecular transporters and development of SWNT-assisted cancer therapies.
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