期刊
NANO LETTERS
卷 10, 期 12, 页码 5109-5115出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl1036098
关键词
Nanothermometry; two-photon microscopy; quantum dots; HeLa cancer cell; thermal sensing; fluorescence imaging
类别
资金
- Universidad Autonoma de Madrid and Comunidad Autonoma de Madrid [CCG087-UAM/MAT-4434, S2009/MAT-1756]
- Spanish Ministerio de Educacion y Ciencia [MAT 2007-64686, MAT2010-16161]
- Banco Santander-CEAL-UAM project
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Gouvernement du Quebec, Ministere du Developpement economique, de l'Innovation et de l'Exportation
- Spanish Minsterio de Educacion [PR2009-0040]
The technological development of quantum dots has ushered in a new era in fluorescence bioimaging, which was propelled with the advent of novel multiphoton fluorescence microscopes. Here, the potential use of CdSe quantum dots has been evaluated as fluorescent nanothermometers for two-photon fluorescence microscopy. In addition to the enhancement in spatial resolution inherent to any multiphoton excitation processes, two-photon (near-infrared) excitation leads to a temperature sensitivity of the emission intensity much higher than that achieved under one-photon (visible) excitation. The peak emission wavelength is also temperature sensitive, providing an additional approach for thermal imaging, which is particularly interesting for systems where nanoparticles are not homogeneously dispersed. On the basis of these superior thermal sensitivity properties of the two-photon excited fluorescence, we have demonstrated the ability of CdSe quantum dots to image a temperature gradient artificially created in a biocompatible fluid (phosphate-buffered saline) and also their ability to measure an intracellular temperature increase externally induced in a single living cell.
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