期刊
NANO LETTERS
卷 9, 期 9, 页码 3258-3261出版社
AMER CHEMICAL SOC
DOI: 10.1021/nl901517b
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资金
- Cancer Center for Nanotechnology Excellence (NCI-CCNE)
- LUNGevity Foundation-American Cancer Society
- NATIONAL CANCER INSTITUTE [U54CA119341] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP1OD000285] Funding Source: NIH RePORTER
We demonstrate a composite nanomaterial, termed an aptamer nano-flare, that can directly quantify an Intracellular analyte in a living cell. Aptamer nano-flares consist of a gold nanoparticle core functionalized with a dense monolayer of nucleic acid aptamers with a high affinity for adenosine triphosphate (ATP). The probes bind selectively to target molecules and release fluorescent reporters which indicate the presence of the analyte. Additionally, these nanoconjugates are readily taken up by cells where their signal intensity can be used to quantity intracellular analyte concentration. These nanoconjugates are a promising approach for the intracellular quantification of other small molecules or proteins, or as agents that use aptamer binding to elicit a biological response in living systems.
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