Application of one-step reverse transcription loop mediated isothermal amplification (reverse transcription LAMP) for rapid detection of fungal gene expression in pure culture mycelia and in planta

A novel application for reverse transcription loop-mediated isothermal amplification (reverse transcription LAMP) was developed which enables rapid, accurate and inexpensive detection of gene expression in pure culture mycelia with no previous mRNA isolation necessary as well as in infected plant tissue after mRNA isolation. Usefulness of the method was demonstrated by detection of mRNA templates of the Fusarium graminearum housekeeping genes coding for Ef1 alpha and beta-tubulin, in mycelia and in planta. (C) 2014 The Mycological Society of Japan. Published by Elsevier B. V. All rights reserved.