Mycorrhiza analyses in New Zealand truffiSres reveal frequent but variable persistence of Tuber melanosporum in co-existence with other truffle species

This study compiles the results from an examination of mycorrhizae on root samples from Tuber melanosporum truffiSres in New Zealand. Samples were taken over 5 years from 328 trees in 43 truffiSres established with nursery-inoculated trees. Mycorrhizae were analysed using a combination of morphological and molecular techniques, focusing on the identification of Tuber species. Results show that 49% of the trees, and nearly 90% of the truffiSres, retained T. melanosporum mycorrhizae up to 21 years after planting. Tuber mycorrhizae with spiky cystidia were found on 26.9% of the tested trees: Tuber brumale (5.5%), Tuber maculatum (10.7%), and unidentified Tuber species (10.7%), and were detected in 67% of the truffiSres tested. T. brumale was found in 28% and T. maculatum in 35% of the truffiSres. In 56% of the truffiSres, T. melanosporum was found to occur with spiky Tuber species. The existence of T. brumale and T. maculatum in the same truffiSre was recorded only once. Forty-four percent of trees examined had Scleroderma-like (SCL) mycorrhizae and 50% of trees hosted other ectomycorrhizal species (OE). For all categories of mycorrhizal species examined, the variation between truffiSres was greater than variation within each truffiSre. Overall results indicate that Corylus avellana tends to be more receptive to mycorrhizae of Tuber species than Quercus robur but is not necessarily more productive. In productive truffiSres, Q. robur appears to host SCL mycorrhizae more often than C. avellana. This is the first study of its scale to analyse the mycorrhizal species associated with T. melanosporum truffiSres in the Southern Hemisphere.