期刊
MYCOPATHOLOGIA
卷 173, 期 1, 页码 1-11出版社
SPRINGER
DOI: 10.1007/s11046-011-9456-0
关键词
Sporothrix schenckii; Dimorphism; 2DE; Protein profile; DRK1 histidine kinase
类别
资金
- National Natural Science Foundation of China [81000699]
Sporotrichosis is a common cutaneous mycosis caused by the dimorphic fungus Sporothrix schenckii, which exhibits a temperature-dependent dimorphic switch. At 25A degrees C, it grows in a mycelial phase, while at 37A degrees C, it forms unicellular yeast cells. The formation of yeast cells was thought to be a requisite for the pathogenicity of S. schenckii. To identify fragments that might be related to morphogenesis, whole-cell proteins from the mold and early yeast stages of S. schenckii were analyzed using 2DE. Among thousands of protein molecules displayed, more than 300 showed a differential expression between the two phases. In particular, 24 yeast-specific proteins were identified using MALDI-TOF/MS. One of the most interesting proteins was a hybrid histidine kinase, DRK1, a global regulator of dimorphism and virulence in Blastomyces dermatitidis and Histoplasma capsulatum that was abundant in the yeast phase. Our study introduced a new approach to study dimorphism in S. schenckii, and the data may help us better understand the molecular mechanisms of phase transition.
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