期刊
MYCOPATHOLOGIA
卷 169, 期 6, 页码 445-449出版社
SPRINGER
DOI: 10.1007/s11046-010-9284-7
关键词
Fungal genomic DNA; FTA technology; PCR; DNA extraction; Molecular identification
类别
Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA(R) filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.
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