期刊
MYCOLOGICAL PROGRESS
卷 10, 期 2, 页码 207-218出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11557-010-0690-5
关键词
Coprophilous fungi; Enzymes; Filamentous fungi; Hydrolases; Biotechnology
类别
资金
- Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia
The extracellular enzymes of seven fungal strains isolated from koala faeces have been comprehensively characterised for the first time, revealing potential for biotechnological applications. The fungal isolates were grown in a hydrolase-inducing liquid medium and the supernatants were analysed using enzyme assays and zymogram gels. Temperature and pH profiles were established for xylanase (EC 3.2.1.8 endo-1,4-beta-xylanase), mannanase (EC 3.2.1.78 mannan endo-1,4-beta-mannosidase), endoglucanase (EC 3.2.1.4 cellulase), beta-glucosidase (EC 3.2.1.21 beta-glucosidase), amylase (EC 3.2.1.1 alpha-amylase), lipase (EC 3.1.1.3 triacylglycerol lipase) and protease (EC 3.4 peptidase) activities. Comparisons were made to the high-secreting hypercellulolytic mutant strain Trichoderma reesei RUT-C30 and the wild-type T. reesei QM6a. The isolates from koala faeces Gelasinospora cratophora A10 and Trichoderma atroviride A2 were good secretors of total protein and heat-tolerant enzymes. Doratomyces stemonitis C8 secreted hemicellulase(s), endoglucanase(s) and beta-glucosidase(s) with neutral to alkaline pH optimums. A cold-tolerant lipase was secreted by Mariannaea camptospora A11. The characteristics displayed by the enzymes are highly sought after for industrial processes such as the manufacture of paper, detergents and food products. Furthermore, the enzymes were produced at good starting levels that could be increased further by strain improvement programs.
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