期刊
MYCOLOGIA
卷 106, 期 1, 页码 43-56出版社
TAYLOR & FRANCIS INC
DOI: 10.3852/13-032
关键词
Botrytis sinoviticola; China; gray mold; molecular identification; table grapes
类别
资金
- Fundamental Research Funds for the Central Universities [2011QC087]
- Special Fund for Agro-scientific Research in the Public Interest [201303025]
Seventy-five isolates of Botrytis collected from table grapes (Vitis vinifera) with gray mold symptoms in China were identified based on morphocultural characteristics on potato dextrose agar (20 C) and/or phylogenetic analysis using the sequences of three nuclear genes (G3PDH, HSP60, RPB2). Isolates of different species of Botrytis were compared with fenhexamid sensitivity, Bc-hch gene-RFLP haplotyping and pathogenicity to V. vinifera. The 75 isolates comprise two species, B. cinerea (63 isolates) and an undescribed Botrytis sp. (12 isolates) described here as Bohytis sinoviticola Zhang et al. sp., nov. Both B. sinoviticola (Bs) and B. cinerea (Bc) were found to have 20 C optimum for mycelial growth and 25 C for conidial germination. Sensitivity to fenhexamid was significantly greater (P < 0.05) for Bc (EC50 = 0.04 +/- 0.01 mu g mL(-1)) than for Bs (EC50 = 0.08 +/- 0.02 mu g mL(-1)). Digestion of the PCR amplicons of the Bc-hch gene with Hha I generated two haplotypes, Group I haplotype for Bs and Group II haplotype for Bc. Bs infected table grapes (leaves, berries) only through wounds, whereas Bc infected both injured and non-injured tissues of table grapes. This study suggests that Bs is a cryptic species sympatric with Bc on table grapes in China.
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