期刊
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
卷 752, 期 1, 页码 10-24出版社
ELSEVIER
DOI: 10.1016/j.mrrev.2012.08.001
关键词
Migration; Invasion; Spheroid; Migration assay; Invasion assay; Extracellular matrix
资金
- European Community [FP6 036097-2]
- Osterreichische Nationalbank [P13423]
- Herzfelder'sche Familienstiftung
- NO Forschungs-und Bildungsges.m.b.H. (NFB)
Determining the migratory and invasive capacity of tumor and stromal cells and clarifying the underlying mechanisms is most relevant for novel strategies in cancer diagnosis, prognosis, drug development and treatment. Here we shortly summarize the different modes of cell travelling and review in vitro methods, which can be used to evaluate migration and invasion. We provide a concise summary of established migration/invasion assays described in the literature, list advantages, limitations and drawbacks, give a tabular overview for convenience and depict the basic principles of the assays graphically. In many cases particular research problems and specific cell types do not leave a choice for a broad variety of usable assays. However, for most standard applications using adherent cells, based on our experience we suggest to use exclusion zone assays to evaluate migration/invasion. We substantiate our choice by demonstrating that the advantages outbalance the drawbacks e.g. the simple setup, the easy readout, the kinetic analysis, the evaluation of cell morphology and the feasibility to perform the assay with standard laboratory equipment. Finally, innovative 3D migration and invasion models including heterotypic cell interactions are discussed. These methods recapitulate the in vivo situation most closely. Results obtained with these assays have already shed new light on cancer cell spreading and potentially will uncover unknown mechanisms. (C) 2012 Elsevier B.V. All rights reserved.
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