期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 756, 期 1-2, 页码 14-20出版社
ELSEVIER
DOI: 10.1016/j.mrgentox.2013.05.006
关键词
DNA damage; DNA repair; Laser; NHEJ; BER
资金
- Science and Technology Facilities Council Biomed Network [HNB3003]
- Medical Research Council [MC_PC_12001]
- MRC [MC_PC_12001] Funding Source: UKRI
- Medical Research Council [MC_PC_12001] Funding Source: researchfish
The formation of DNA lesions. poses a constant threat to cellular stability. Repair of endogenously and exogenously produced lesions has therefore been extensively studied, although the spatiotemporal dynamics of the repair processes has yet to be fully understood. One of the most recent advances to study the kinetics of DNA repair has been the development of laser microbeams to induce and visualize recruitment and loss of repair proteins to base damage in live mammalian cells. However, a number of studies have produced contradictory results that are likely caused by the different laser systems used reflecting in part the wavelength dependence of the damage induced. Additionally, the repair kinetics of laser microbeam induced DNA lesions have generally lacked consideration of the structural and chemical complexity of the DNA damage sites, which are known to greatly influence their reparability. In this review, we highlight the key considerations when embarking on laser microbeam experiments and interpreting the real time data from laser microbeam irradiations. We compare the repair kinetics from live cell imaging with biochemical and direct quantitative cellular measurements for DNA repair. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据