期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 742, 期 1-2, 页码 61-65出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mrgentox.2011.12.003
关键词
Silver nanoparticle; Genotoxicity; Comet assay; Hydrogen peroxide; Free radicals
资金
- Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India
Nano-silver (Nano-Ag) particles were synthesized and then characterized using transmission electron microscopy (TEM) and X-ray diffractometry. TEM showed that Nano-Ag were spherical in shape and their size ranged from 40 to 60 nm. X-ray diffractometry indicated that the sample was crystalline and had a face centered cubic structure of pure silver. Genotoxicity of this Nano-Ag was evaluated in human peripheral blood cells using the alkaline comet assay. Results indicated that Nano-Ag (50 and 100 mu g/mL) caused DNA damage following a 3 h treatment. Subsequently, a short treatment of 5 min also showed DNA damage. In conclusion, we have shown that the synthesized Nano-Ag induced DNA damage in human peripheral blood cells as detected by the alkaline comet assay. Results further indicated that treatment of cells with Nano-Ag in the presence of hydrogen peroxide did not induce any DNA damage. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据