期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 726, 期 1, 页码 1-7出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mrgentox.2011.07.011
关键词
Mycotoxin; Biotest; Screening; Biodegradation; Aflatoxin-B1
资金
- NKTH [TECH_08-A3/2-2008-0385 (OM-00234/2008)]
To monitor cytotoxic and genotoxic effects of aflatoxin, a luminescent assay employing Aliivibrio fischeri as a test organism and a colorimetric assay based on the SOS-Chromotest were adapted to our needs. The aim of this method-developing work was to be able to select - from a collection of environmental isolates - microbes that degrade aflatoxin without production of harmful intermediates and by-products, in a fast and cost-effective way. By the combination of the two modified assays, microbes that met these criteria have been successfully selected. Among thirty-three isolates, the strain Rhodococcus rhodochrous NI2 proved to be the best aflatoxin-B1-degrading microbe, with the weakest harmful biological effects throughout aflatoxin-B1-degradation. Our findings underline the necessity to employ bio-tests in biodegradation assays, as cytotoxicity and/or genotoxicity may occur even after substantial degradation of the toxins. (C) 2011 Elsevier B.V. All rights reserved.
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